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rabbit anti mouse tollip (Boster Bio)

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Boster Bio rabbit anti mouse tollip

Figure 1. Effect of XBJ on the mRNA expression of <t>Tollip,</t> IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.

Rabbit Anti Mouse Tollip, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti mouse tollip/product/Boster Bio
Average 90 stars, based on 1 article reviews

rabbit anti mouse tollip - by Bioz Stars, 2026-04

90/100 stars

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1) Product Images from "Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis."

Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

Journal: International journal of molecular medicine

doi: 10.3892/ijmm.2014.1943

Figure 1. Effect of XBJ on the mRNA expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.

Figure Legend Snippet: Figure 1. Effect of XBJ on the mRNA expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.

Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Control

Figure 2. Administration of XBJ led to increased expression levels of Tollip mRNA, and inhibition of TLR4, NF-κB65 and TRAF6 mRNA expression in lung tissue in CLP-ALI mice. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Statistical summary of the densitometric analysis of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 expression in the four rat groups. Data are presented as mean ± stan dard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡P<0.05 and ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

Figure Legend Snippet: Figure 2. Administration of XBJ led to increased expression levels of Tollip mRNA, and inhibition of TLR4, NF-κB65 and TRAF6 mRNA expression in lung tissue in CLP-ALI mice. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Statistical summary of the densitometric analysis of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 expression in the four rat groups. Data are presented as mean ± stan dard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡P<0.05 and ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

Techniques Used: Expressing, Inhibition, Reverse Transcription Polymerase Chain Reaction, Control

Figure 4. Administration of XBJ enhanced the expression of Tollip protein protein, and inhibition TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Statistical summary of the densitometric analysis of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡﹡P<0.01 vs. the normal control group and sham operation group. ﹟P<0.05, ﹟﹟P<0.01 vs. the control group.

Figure Legend Snippet: Figure 4. Administration of XBJ enhanced the expression of Tollip protein protein, and inhibition TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Statistical summary of the densitometric analysis of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡﹡P<0.01 vs. the normal control group and sham operation group. ﹟P<0.05, ﹟﹟P<0.01 vs. the control group.

Techniques Used: Expressing, Inhibition, Western Blot, Standard Deviation, Control

Figure 3. Administration of XBJ enhanced the expression of Tollip protein, and inhibition of TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Representative western blots show the level of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. A, normal control group; B, sham operation group; C, control group; D, treatment group.

Figure Legend Snippet: Figure 3. Administration of XBJ enhanced the expression of Tollip protein, and inhibition of TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Representative western blots show the level of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. A, normal control group; B, sham operation group; C, control group; D, treatment group.

Techniques Used: Expressing, Inhibition, Western Blot, Control

Figure 5. Administration of XBJ upregulated Tollip positive protein in lung tissue in CLP-ALI mice. Groups of mice were challenged with CLP and treated with XBJ 24 h later. Tollip positive protein levels of lung tissue were determined using immunohistochemistry and the average proportion of positive expres sion in each field was counted using the true color multi-functional cell image analysis management system. Values are expressed as mean ± SD; ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

Figure Legend Snippet: Figure 5. Administration of XBJ upregulated Tollip positive protein in lung tissue in CLP-ALI mice. Groups of mice were challenged with CLP and treated with XBJ 24 h later. Tollip positive protein levels of lung tissue were determined using immunohistochemistry and the average proportion of positive expres sion in each field was counted using the true color multi-functional cell image analysis management system. Values are expressed as mean ± SD; ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

Techniques Used: Immunohistochemistry, Functional Assay, Control

Image Search Results

Journal: International journal of molecular medicine

Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

doi: 10.3892/ijmm.2014.1943

Figure Lengend Snippet: Figure 1. Effect of XBJ on the mRNA expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.

Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Control

Journal: International journal of molecular medicine

Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

doi: 10.3892/ijmm.2014.1943

Figure Lengend Snippet: Figure 2. Administration of XBJ led to increased expression levels of Tollip mRNA, and inhibition of TLR4, NF-κB65 and TRAF6 mRNA expression in lung tissue in CLP-ALI mice. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Statistical summary of the densitometric analysis of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 expression in the four rat groups. Data are presented as mean ± stan dard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡P<0.05 and ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Expressing, Inhibition, Reverse Transcription Polymerase Chain Reaction, Control

Journal: International journal of molecular medicine

Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

doi: 10.3892/ijmm.2014.1943

Figure Lengend Snippet: Figure 4. Administration of XBJ enhanced the expression of Tollip protein protein, and inhibition TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Statistical summary of the densitometric analysis of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡﹡P<0.01 vs. the normal control group and sham operation group. ﹟P<0.05, ﹟﹟P<0.01 vs. the control group.

Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Expressing, Inhibition, Western Blot, Standard Deviation, Control

Journal: International journal of molecular medicine

Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

doi: 10.3892/ijmm.2014.1943

Figure Lengend Snippet: Figure 3. Administration of XBJ enhanced the expression of Tollip protein, and inhibition of TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Representative western blots show the level of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. A, normal control group; B, sham operation group; C, control group; D, treatment group.

Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Expressing, Inhibition, Western Blot, Control

Journal: International journal of molecular medicine

Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

doi: 10.3892/ijmm.2014.1943

Figure Lengend Snippet: Figure 5. Administration of XBJ upregulated Tollip positive protein in lung tissue in CLP-ALI mice. Groups of mice were challenged with CLP and treated with XBJ 24 h later. Tollip positive protein levels of lung tissue were determined using immunohistochemistry and the average proportion of positive expres sion in each field was counted using the true color multi-functional cell image analysis management system. Values are expressed as mean ± SD; ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Immunohistochemistry, Functional Assay, Control

FIGURE 1. TOLLIP interrupts time-dependent expression of TLRs on M. A, cells were cultured with (black line) or without (shaded) HlyA and analyzed by flow cytometry for the expression of TLR1, -2, -4, and -6 at time points shown between 2–48 h. B, cell lysate was prepared after 10 h of incubation of M with andwithoutHlyA,electrophoresed,andimmunoblottedfordetectionofTOLLIP.Theblotwasreprobedwithanti--actinAbtoensureequalloadingofprotein in both lanes. C, M of C57BL/6 mice were cultured in absence and presence of HlyA or with anti-TLR4 Ab plus HlyA and analyzed for expression of TLR2 and -6. Up-regulation of TLR2 was also analyzed on M of C3H/HeJ mice treated with or without HlyA. The data given were obtained in one of three representative experiments. *, p 0.05.

Journal: Journal of Biological Chemistry

Article Title: Hemolysin Induces Toll-like Receptor (TLR)-independent Apoptosis and Multiple TLR-associated Parallel Activation of Macrophages

doi: 10.1074/jbc.m111.241851

Figure Lengend Snippet: FIGURE 1. TOLLIP interrupts time-dependent expression of TLRs on M. A, cells were cultured with (black line) or without (shaded) HlyA and analyzed by flow cytometry for the expression of TLR1, -2, -4, and -6 at time points shown between 2–48 h. B, cell lysate was prepared after 10 h of incubation of M with andwithoutHlyA,electrophoresed,andimmunoblottedfordetectionofTOLLIP.Theblotwasreprobedwithanti--actinAbtoensureequalloadingofprotein in both lanes. C, M of C57BL/6 mice were cultured in absence and presence of HlyA or with anti-TLR4 Ab plus HlyA and analyzed for expression of TLR2 and -6. Up-regulation of TLR2 was also analyzed on M of C3H/HeJ mice treated with or without HlyA. The data given were obtained in one of three representative experiments. *, p 0.05.

Article Snippet: The cell lysates were electrophoresed, Western-blotted, and incubated overnight with rabbit antimouse TOLLIP mAb (Santa Cruz Biotechnology), anti-mouse Bcl-xL, caspase-6, or caspase-7 Ab (Cell Signaling Technology).

Techniques: Expressing, Cell Culture, Flow Cytometry, Incubation

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1) Product Images from "Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis."

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